Mascot is a powerful search engine that uses mass spectrometry data to identify proteins from primary sequence databases. Mascot is unique in that it integrates all of the proven methods of searching, including:
- Peptide Mass Fingerprinting, in which the only experimental data are peptide mass values
- Sequence Query, in which peptide mass data are combined with amino acid sequence and composition information
- MS/MS Ion Search, which uses uninterpreted MS/MS data from one or more peptides
For details on the search algorithm as well as a freely accessible web version (with some limitations on the number of queries that can be submitted at any time) please visit the MatrixScience web site.
Mascot is installed on a quad-core Linux server and can be accessed at http://research.cchmc.org/mascot/. You will need to obtain a user name and password to access this search engine.